A Targeted, Medium-High Density, Multiplexed Genotyping Platform for Marker-Assisted Plant Breeding

Marker-assisted breeding – a technique by which trait selection is aided by linking the trait with an associated genetic marker – requires access to high-throughput, low-cost genotyping of a genome-wide sample of DNA polymorphisms. Next-generation sequencing (NGS) approaches are often more cost-effective for large-scale applications compared to SNP arrays.

During this webinar, Mitchell J. Feldmann, postdoctoral researcher at the University of California, Davis, will discuss how targeted, medium-high density, multiplexed genotyping platforms can avoid several shortcomings of both SNP arrays and NGS-based genotyping by sequencing for heterozygous, outbred cultivated strawberry.

Learn how a high-throughput, medium-high density genotyping platform populated with 5,000 sub-genome specific SNPs connected to the 50K FanaSNP Axiom array for cultivated strawberries was designed and tested. Markers were selected to best reproduce genomic relationship matrices calculated with the 50K FanaSNP array and to cover the linkage landscape in a large diversity panel of exotic, heirloom, and modern cultivars.

Attendees will learn about:

• Marker-assisted breeding.
• Platform development and testing.
• The analyses enabled by a high level of connectivity across platforms.

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